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KMID : 0644120210340010034
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Journal of Oriental Gynecology
2021 Volume.34 No. 1 p.34 ~ p.47
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Anti-inflammatory Effect and Mechanism of Citri Reticulatae Viride Pericarpium Water Extract
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Ahn Tae-Seok
Hwang Deok-Sang
Lee Jin-Moo
Jang Jun-Bock
Lee Chang-Hoon
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Abstract
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Objective: This study was designed to examine anti-inflammatory effect and mechanism of Citri Reticulatae Viride Pericarpium water extract (CRE).
Methods: Cell cytotoxicity was tested with RAW 264.7 cells. To investigate anti-inflammatory effect of CRE in lipopolysaccharide (LPS)-induced RAW 264.7 cell, we measured nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10). In addition, mitrogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) were examined by western blotting in LPS-induced RAW 264.7 cell with treated CRE.
Results: In cytotoxicity analysis, CRE does not affect cell cytotoxicity. As compared with the control group, the expression of NO, PGE2, TNF-α, IL-6 were significantly decreased, and IL-10 was significantly increased in LPS-induced RAW 264.7 cell with treated CRE. As a result of Western blotting, there was concentration-dependent inhibition of pp38, pERK in MAPK pathway and significant reduction of pp65 in the NF-κB pathway.
Conclusion: CRE might have anti-inflammatory effect in LPS-induced macrophages by promoting the production of IL-10.
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KEYWORD
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Citri Reticulatae Viride Pericarpium, Anti-inflammatory, Lipopolysaccharide, Macrophage, Interleukin-10
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